Genes
Oligo Pools
NGS
Variant Libraries
Antibodies
IVDR Solutions
Technical Note
Protocol
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
High Performance Multiplexed Target Enrichment Sequencing from FFPE Tissues
Presented at ASHG 2019: Twist Target Enrichment workflows and Covaris DNA extraction deliver high performance targeted sequencing.
Library construction for Next Generation Sequencing (NGS) using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide distribution of sample quality. Differences in formalin fixation methods, storage conditions, and age lead to cross linked and/or degraded nucleic acid and inconsistent extraction yields. Therefore, FFPE extraction and library construction methods must be carefully considered for target enrichment applications. In collaboration, Covaris and Twist Bioscience demonstrate a complete library preparation and target enrichment solution that generates ready-to-sequence multiplexed libraries directly from FFPE tissue of various qualities.
