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Application Note
Webinar
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Prioritization of Antimicrobial Targets by CRISPR-based Oligo Recombineering
Prioritization of Antimicrobial Targets by CRISPR-based Oligo Recombineering
Presented by
Matthew Child
Group Leader, Imperial College London
RECORDED AT Writing the Future of Infectious Disease
Covered in this Webinar
Development of CRISPR-based Oligo Recombineering (CORe) to prioritize reactive AAs according to contribution to protein function
How CORe couples protein sequence and function with biological fitness, outperforming traditional genetic workflow efficiency
Results including discovery of reactive cysteines decorating the ribosome of apicomplexan parasite Toxoplasma gondii
Chemoproteomic technologies can mine chemically-accessible amino acids via intrinsic reactivity toward electrophilic probes. However these approaches cannot discern which reactive residues contribute to protein function and therefore prioritize for drug discovery.
In this webinar:
- Development of CRISPR-based Oligo Recombineering (CORe) to systematically prioritize reactive amino acids according to their contribution to protein function.
- How CORe couples protein sequence and function with biological fitness, outperforming traditional genetic workflow efficiency by orders-of-magnitude.
- Results including discovery of reactive cysteines decorating the ribosome of apicomplexan parasite Toxoplasma gondii that were found to be critical for parasite growth, with target-based screening validating apicomplexan translation machinery as a novel target for covalent ligand development.
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